By Tadhg P. Begley
The Wiley Encyclopedia of Chemical Biology is an authoritative new paintings whose target is to light up the the most important function of chemistry and chemical suggestions within the existence sciences. The encyclopedia will undertake an inclusive editorial technique, encompassing basic and blue-sky technological know-how in addition to these components of study that experience extra quick scientific or advertisement functions. The scope and constitution of the paintings will mirror the multidimensional personality of chemical biology, focusing particularly at the primary technological know-how of organic constructions and structures, using chemical and organic ideas to clarify that technology, and the functions of this information in components as diversified as drug discovery, sensor know-how, and catalysis.Major themes parts lined within the encyclopedia:Chemical perspectives of BiologyBiomolecules in the CellChemistry of organic approaches and SystemsChemical Biology of mobile CompartmentsSynthetic Molecules as instruments for Chemical BiologyTechnologies and methods in Chemical BiologyApplications of Chemical Biology
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Currently, colorimetric or fluorometric assays are the most popular and convenient screening methods, whereby the positive variants can be easily identified by visual check or by measuring UV-Vis absorbance or fluorescence using a plate reader. However, they are not available for all enzymes. Other generic screening tools, such as HPLC, capillary electrophoresis, and thermistor arrays, have also been applied to engineering of enzymes (21). To address the low throughput limitation associated with most screening methods, various fluorescence-activated cell sorting (FACS) based screening methods have been developed.
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Crit. Rev. Ther. Drug Carrier Sys. 2006;23:437–495. Patri AK, Kukowska-Latallo JF, Baker Jr. Targeted drug delivery with dendrimers: comparison of the release kinetics of covalently conjugated drug and non-covalent drug inclusion complex. Adv. Drug Delivery Rev. 2005;57:2203–2214. Hamidi M, Zarrin A, Foroozesh M, Mohammadi-Samani S. Applications of carrier erythrocytes in delivery of biopharmaceuticals. J. Control. Rel. 2007;118:145–160. Rossi L, Serafini S, Pierige F, Antonelli A, Cerasi A, Fraternale A, Chiarantini L, Magnani M.
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