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ACS Symposium Series; American Chemical Society: Washington, DC, 1990. - -6 - 44 IMMUNOASSAYS FOR TRACE CHEMICAL ANALYSIS p o s i t i v e only with the 1-2 TEST and 2 p o s i t i v e by the culture method. The s e l e c t i v e enrichment modification enhanced i t s r e l i a b i l i t y for Salmonella detection i n foods and feeds. The manufacturer's current procedure s p e c i f i e s a s e l e c t i v e enrichment step. Although the r e l i a b i l i t y of the 1-2 TEST had been improved greatly, when inoculated from the enrichment broth, i t unfortunately prolonged the time for a n a l y s i s by 24 hours.

Clin. Chem. Clin. Biochem. 1980, 18, 197-208. 15. ; Dorn, E . ; Beyermann, K. J . Agric. Food Chem. 1984, 32, 734-741. 16. ; Jensen, R. ; Vanderlaan, M. J . Agric. Food Chem. 1989, 37, 834-839. 17. O. J . Agric. Food Chem. 1982, 30, 572-580. 18. N. Anal. Chem. 1986, 58, 1866-1873. 19. D In Analytical Methods for Pesticides an Analytical Technigues Press: New York, 1989, pp 217-263. 20. D. J . Agric. Food Chem. 1982, 30, 943-948. 21. D. J . Agric. Food Chem. 1982, 30, 949957. 22. D. J . Agric. Food Chem.

One,of the key analyses performed i n m i c r o b i o l o g i c a l t e s t i n g laboratories i s the detection of Salmonella. A l l known Salmonella spp are pathogenic to man and/or animals and cause the well-known Salmonellosis i n f e c t i o n . Even though federal regulations p r o h i b i t i n g the presence of Salmonella i n foods are i n place, the recovery of t h i s organism from a p a r t i c u l a r food commodity may be q u i t e difficult. Surveillance of salmonellae by the 6-step conventional culture method (6) i s t e s t requiring a minimu an a d d i t i o n a l 24 hours.

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