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By Gregory Pincus, Takeshi Nakao, James F. Tait

Steroid Dynamics compiles papers offered on the Symposium at the Dynamics of Steroid Hormones held in Tokyo, in may possibly 1965.

This compilation discusses the impression of LH and ACTH on construction and excretion of testosterone in vivo, estrogen receptors in objective tissues, and impact of morphine on corticoid creation. The binding of steroids to follicular fluid proteins, conjugation and excretion of aldosterone, and lively ldl cholesterol pool for corticodogenesis within the rat adrenal also are elaborated. This e-book likewise covers the dynamics of steroid hormone distribution within the physique, biosynthesis of steroids in testicular tissue in vitro, and isotopic dilution tools for the estimation of premiums of secretion of the steroid hormones.

This booklet is an effective reference for endocrinologists, biochemists, and clinical practitioners attracted to steroid dynamics.

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Extra resources for Steroid Dynamics. Proceedings of the Symposium on the Dynamics of Steroid Hormones Held in Tokyo, May, 1965

Example text

T h e capacity of albumin to bind various steroids is t r e m e n d o u s , since in competition experiments w e w e r e unable to show that estrone, estradiol, estriol, testosterone, and progesterone interfered with the binding by albumin of any of the other steroids. 0U .. 8 r F I G . 17. , 1963). Four series of experiments are shown: 0 , 0 . 85; A, 0 . 35. T h e curve o b e y s the Scatchard equation w h e n calculated on the basis of o n e strong site and about 2 0 lesser sites. S is the molar concentration of unbound steroid and r is the moles of steroid bound per mole of albumin.

In o r d e r to obtain the distribution of e n d o g e n o u s Cortisol b e t w e e n albumin-bound, transcortin-bound, and u n b o u n d Cortisol, the c o n c e p t underlying the construction of the Scatchard plot m u s t be e x p a n d e d to include the u n b o u n d steroid. A slight change of definitions permits us to plot a c u r v e that is the sum of t h r e e straight lines, o n e for binding to albumin, o n e for binding to transcortin, and o n e for u n b o u n d steroid, which w e shall redefine as " s a l i n e - b o u n d " steroid: "Saline-bound" cortistol = unbound Cortisol = u (10) Bound steroid now b e c o m e s the sum of albumin-bound, transcortinbound, and " s a l i n e - b o u n d " steroid.

Against albumin Slaunwhite and Sandberg (1959) Warren and Salhanick (1960) 2. Against saline or buffer (at 9°C) Sandberg et al (1960) Murphy and Pattee (1963) Undiluted plasma 1. Against saline or buffer 2. D o u b l e dialysis technique 3. "Suction dialysis" Daughaday (1958b) Bush (1957) Daughaday et al. (1962) D o e et al. (1960) (B) Dialysis at 37°C Diluted plasma 1. Against albumin Booth et a/. (1961) Warren and Salhanick (1960) 2. Against saline or buffer In high dilutions Slaunwhite and Sandberg (1959) Murphy and Pattee (1963) Undiluted plasma 1.

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