Download Musicofilia by Oliver Sacks PDF

By Oliver Sacks

Oliver Sacks examina l. a. relación con los angeles música de pacientes, gente corriente o músicos profesionales para arrojar una luz insólita sobre ese fenómeno. A través de anomalías como los angeles «amusia» –o incapacidad para sentir los angeles música–, el hipermusical síndrome de Williams –un extraño fenómeno de extrema sociabilidad–, las alucinaciones musicales o los angeles música como inspiradora de auténtico terror, Sacks elabora un lúcido análisis de cómo l. a. música es un issue clave para crear los angeles identidad humana, ya sea de una manera patógena o como un agente positivo a los angeles hora de tratar el Parkinson, l. a. demencia, el síndrome de Tourette, l. a. encefalitis o los ataques de lóbulo temporal. Sacks se revela de nuevo como un espléndido narrador, con el humor, l. a. erudición y los angeles vastísima cultura científica y humanista a que nos tiene acostumbrados.

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As with many biochemicals, repeated freeze-thaw cycles and unfavorable chemical conditions can also degrade fluorescently labeled nucleotides. To maximize signal by promoting efficient probe labeling and hybridization, fluorescent compounds should be protected from light, and the manufacturers’ handling instructions should be followed exactly. Pickett, Basarsky, Verdnik, and Wellis 34 Data Acquisition Although signal-to-noise considerations spread across sample preparation, data acquisition, and data extraction, the overall sensitivity of the hardware portion of this process should not be based on any single specification.

FILTERS AND MIRRORS Fluorescence-based microarray imaging relies on the fact that one can illuminate a fluorophore with one wavelength of light, and that the fluorophore then emits light at a longer wavelength. This effect, known as the Stokes shift, is a fundamental property of fluores- Pickett, Basarsky, Verdnik, and Wellis 40 cence microscopy (see Rost, 1991). However, fluorophores are not tuned to a single wavelength, but instead absorb and emit across a range of wavelengths. A properly designed scanner must optimize the delivery and collection of only the appropriate wavelengths of light.

10. Scan array at wavelengths appropriate for Cy3 and Cy5. For other protocols see: 1. html/) 2. org) 3. microarray) J. P. Gregg 28 References Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, et al. (2000). The genome sequence of Drosophila melanogaster. Science 287(5461): 2185–95. Chee M, Yang R, Hubbell E, Berno A, Huang XC, Stern D, Winkler J, Lockhard DJ, Morris MS, and Foder SP (1996). Accessing genetic information with high-density DNA arrays. Science 274(5287): 610–14.

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