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By J. D. Bancroft (Auth.)

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Additional resources for Histochemical Techniques

Sample text

A method of freeze drying based on the use of a dry gas moving over the frozen tissues to remove the water evaporating from them was reported by Treffenburg (1953), Jansen (1954) and K r a m e r and Hill (1956). Some provision for drying the air and maintaining the low temperature of the air and specimens was required during dehydration. K r a m e r and Hill used a mixture of carbon dioxide snow and cellulose in a vacuum flask to cool the chamber and to supply a stream of dry gas. T h e drying time claimed for this type of freeze dryer is 10-24 h for small pieces of tissue.

Burs tone (1956) used this method to demonstrate hydrolytic enzymes in freeze dried material. The subject is more fully discussed in Chapter 15. An acrylic resin such as methacrylate, used m u c h as the electron microscopists use it, is sometimes preferred as an embedding medium when thin or very thin sections are required. Polymerization of methacrylate or other resins is achieved by heating at 50°C for eighteen hours or at 60°C overnight, and for this reason, the method is only used for specialized techniques.

41 GRYOSTATS satisfactorily frozen on the block holder by this technique, although the freezing is neither rapid nor even enough for most histochemical methods. T h e big advantage of these sprays is their simplicity and easy availability, with minimal storage problems. Freezing of m u s c l e Muscle is the most difficult of routine biopsy tissues to freeze without serious artefact, and to obtain satisfactory results three conditions must be fulfilled. First, the piece of muscle selected must be small (not exceeding 5 m m X 4 m m ) .

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