By Paolo Gattuso MD, Vijaya B. Reddy MD, Odile David MD, Daniel J. Spitz MD, Meryl H. Haber MD
Differential analysis in Surgical Pathology, second variation, via Paolo Gattuso, MD, Vijaya B. Reddy, MD, Odile David, MD, and Daniel J. Spitz, MD, is skillfully designed that can assist you hopefully signal out your most intricate and difficult situations. overlaying an entire diversity of tumors and tumor-like stipulations in all organ structures, it offers a common highway map to the most standards you have to think about with a purpose to differentiate among a number of strength diagnoses that each one have a truly related visual appeal. Over 1,350 new full-color macro- and microphotographs supply a pragmatic foundation for comparability to what you notice less than the microscope. speedy checklists conceal all diagnostic percentages to ensure not anything falls during the cracks. Full-text on-line entry at expertconsult.com enables you to reference the e-book from any machine and obtain all of its images.A concise, bulleted textual structure allows quickly retrieval of crucial facts.A constant method of prognosis and interpretation expedites reference.Coverage of all suitable ancillary diagnostic suggestions addresses all the investigative contexts had to formulate a correct diagnosis.Expert "pearls" provide sensible tips about what diagnostic standards to contemplate or exclude.A finished, but conceivable dimension allows quickly consultation.Online entry at expertconsult.com permits you to quick seek advice the ebook from any computing device and obtain all of its images.Over 1,350 new full-color macro- and microphotographs supply a practical foundation for comparability to what you notice lower than the microscope. Immunohistochemical and molecular recommendations all through aid you evaluate the entire newest diagnostic concerns in a single place.Expanded assurance of non-neoplastic entities assists you in spotting benign lesions which may mimic the looks and features of malignant ones.Extensive updates comprise the most recent type schemes and correct diagnostic innovations. A brand-new, color-coded format highlights key issues extra basically and is helping you switch to the sections you wish extra speedily.Your buy entitles you to entry the website till the following variation is released, or till the present variation is not any longer provided on the market via Elsevier, whichever happens first. Elsevier reserves definitely the right to supply an appropriate alternative product (such as a downloadable or CD-ROM-based digital model) should still entry to the website be discontinued.
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Additional resources for Differential Diagnosis in Surgical Pathology: Expert Consult - Online and Print 2nd Edition
San Diego, CA. FDA-cleared TMA tests are available for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycobacterium tuberculosis (APTIMA CT, APTIMA GC, and, AMPLIFIED Mycobacterium tuberculosis Direct Test [MTD] assays, respectively) An FDA-approved TMA qualitative assay for hepatitis C virus (HCV) is also available (VERSANT HCV RNA [distributed by Siemens Healthcare Diagnostics, Deerfield, IL]) — Nucleic acid sequence–based amplification (NASBA) ◆ NASBA is an isothermal amplification technique and can be used for the amplification of a DNA or RNA target.
Following the first PCR cycle, there is (theoretically) a per-cycle doubling in the number of copies of the PCR product. (Modified from Leonard DGB [ed]: Diagnostic Molecular Pathology. ) — Basic PCR method ◆ During the denaturation stage, sample specimen DNA is rendered single-stranded by heating to 94° to 98°C ◆ In the annealing step, oligonucleotide primers hybridize with the target sequences they have been designed to complement. The annealing temperature depends on deoxyribonucleoside triphosphate (dNTP) composition of the primers and is typically in the range of 40° to 60°C ◆ During the extension step (72°C), the annealed primer or target DNA seeds the (5′ → 3′) synthesis by thermostable DNA polymerase of a new DNA strand ◆ DNA amplification is accomplished by repetition of the denaturation, annealing, and extension cycle, 30 to 50 more times ◆ The time period for each of the denaturation, annealing, and extension steps can vary from 10 seconds to more than 1 minute and depends on reaction volume size, amplicon base composition and length, thermostable DNA polymerase activity (about 1000 bp are extended per minute), and thermal cycler hardware specifications — The essential ingredients in a PCR include ◆ DNase or RNase free pure water: final PCR reaction volumes vary from 10 to 50 µL Buffer: pH is typically maintained using a TrisHCl–based buffer.
Clinical applications generally involve PCR amplification of a defined target region followed by sequencing; available tests include — Autosomal recessive polycystic kidney disease (ARPKD) mutation screen — Biotinidase deficiency (BTD) gene analysis — CFTR gene analysis — 21-Hydroxylase (CYP21A2) gene analysis — Dentatorubral-pallidoluysian atrophy (DRPLA) gene analysis — Fabry disease gene analysis — Galactose-1-phosphate uridyltransferase gene (GALT) gene analysis — MLH1 HNPPCC mutation screen — MLH1/MSH2 mutation screen — MSH2 mutation screen — MSH6 mutation screen — Niemann-Pick type C (NPC) mutation screen — Progranulin (GRN) gene analysis — Von Hippel-Lindau disease (VHL) gene analysis — FDA-cleared sequencing assays are available for HIV drug resistance testing (ViroSeq HIV1 Genotyping System, Celera Diagnostics, CA, and TruGene HIV-1 Genotyping and Open Gene DNA Sequencing System, Siemens Healthcare Diagnostics, Deerfield, IL) ● Emerging Methodologies Protein Analytical Methods DNA methylation assays are likely to increase in significance as more is discovered about the importance of epigenetic factors in disease etiology.
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