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By Herbert E. Spiegel

Compliment for the sequence: "Its continuity of pertinence, excellence, and authority continues to be unbroken - a tribute to the skilful modifying and writing concerned. each knowledgeable laboratory employees should have on hand a replica of this volume." --Clinical Chemistry For greater than thirty years, this serial has broadened the technical scope and increased the medical base of medical chemistry. those volumes make clear the parts of molecular biology, informatics, and the tracking of physiological parameters in severe occasions as they pertain to scientific chemistry. every one quantity of Advances in medical Chemistry includes an index, and every bankruptcy contains references.

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586-591 (1995). B4. Bej, A. , Mahbubani, M. , and Atlas, R. , Amplification of nucleic acids by polymerase chain reaction (PCR) and other methods and their applications. Crif. Rev. Biochem. Mol. Biol. 26, 301-334 (1991). B5. , Uhler, M. , Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. Clin. Chem. (Winston-Salem, N . C . ) 41, 1461- 1466 (1995). B6. , Interference of heparin with the polymerase chain reaction.

Interphase cytogenetics of hematological cancer: Comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes. Can. Res. 51, 1959-1967 (1991). P2. , Rosenberg, E. , Keiser, J. , Gross, J. , Ross, A. M. er a / . Nested MOLECULAR BIOLOGY IN CLINICAL BIOCHEMISTRY RI. R2. R3. SI . S2. S3. S4. S5. S6. S7. S8. S9. TI. T2. VI. V2. WI . W2. 37 polymerase chain reaction assay for the detection of cytomegalovims overcomes false positives caused by contamination with fragmented DNA.

They are also present in high copy number organized as tandem repeats of unique I7 1 base-pair sequences that are present in as many as 5000 copies (A2); hence the sensitivity of probes to a-satellite DNA. The second type of human satellite DNA is beta (p)-satellite DNA located in the pericentric heterochromatin of many chromosomes. The third type of human satellite DNA is called classical satellites I, 11, and 111 and is based on short repeats of the base sequence adenine, adenine, thymine, guanine, and guanine (AATGG); it is located in the pericentric heterochromatin of at least chromosomes 1, 9, 15, and 16, and in the long arm of the Y sex chromosome.

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